Derived from chromosome conformation capture (3C) technology, Hi‑C uses intact cell nuclei as the research object to investigate the spatial relationships of entire chromatin DNA across the whole genome. By capturing all DNA–DNA interaction patterns within chromatin, Hi‑C yields high‑resolution information on the three‑dimensional (3D) structure of chromatin.
Integrated multi‑omic analysis with WGS, RNA‑seq, ATAC‑seq, CUT&Tag and other approaches enables the investigation of genome spatial architecture (including compartments, TADs and loops) across diverse species, thereby revealing the 3D genome organization and dissecting the mechanisms underlying tumorigenesis, disease progression and species evolution.
Workflow
Applications
Quantify cross-linked chromatin interactions genome-wide in three-dimensional space
Resolve genome-wide interaction patterns, such as promoter–enhancer interactions
Construct three-dimensional structural models and characterize genome architecture, including compartments, TADs, and loops
Establish a genome-wide interaction map
Assist in improving genome assembly
Construct a genome-wide haplotype map
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